c2c12 murine skeletal muscle cell line Search Results


c2c12 murine skeletal muscle cell line  (Dainippon Sumitomo)
90
Dainippon Sumitomo c2c12 murine skeletal muscle cell line
Distribution of estrogen receptors in various organs and tissues of adult mouse. a Western blot analysis of whole-cell fractions with a specific monoclonal estrogen receptor (ER) antibody. Upper panel immunoreactive bands corresponding to the ER protein (66 kDa). The histogram (lower) indicates the results of densitometric analysis for a 66-kDa band (mean ± SE, n = 4). Relative expression level with respect to the ovary is shown in %. From left to right: skeletal muscle, uterus, lung, adipose tissue, kidney, and myocardium. b Relative expression (to ovary) of whole cell and nonnuclear ER proteins obtained from <t>C2C12</t> mouse myoblasts (n = 3)
C2c12 Murine Skeletal Muscle Cell Line, supplied by Dainippon Sumitomo, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/c2c12 murine skeletal muscle cell line/product/Dainippon Sumitomo
Average 90 stars, based on 1 article reviews
c2c12 murine skeletal muscle cell line - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier

Image Search Results


Distribution of estrogen receptors in various organs and tissues of adult mouse. a Western blot analysis of whole-cell fractions with a specific monoclonal estrogen receptor (ER) antibody. Upper panel immunoreactive bands corresponding to the ER protein (66 kDa). The histogram (lower) indicates the results of densitometric analysis for a 66-kDa band (mean ± SE, n = 4). Relative expression level with respect to the ovary is shown in %. From left to right: skeletal muscle, uterus, lung, adipose tissue, kidney, and myocardium. b Relative expression (to ovary) of whole cell and nonnuclear ER proteins obtained from C2C12 mouse myoblasts (n = 3)

Journal: The Journal of Physiological Sciences : JPS

Article Title: 17β-Estradiol-induced enhancement of estrogen receptor biosynthesis via MAPK pathway in mouse skeletal muscle myoblasts

doi: 10.1007/s12576-009-0023-0

Figure Lengend Snippet: Distribution of estrogen receptors in various organs and tissues of adult mouse. a Western blot analysis of whole-cell fractions with a specific monoclonal estrogen receptor (ER) antibody. Upper panel immunoreactive bands corresponding to the ER protein (66 kDa). The histogram (lower) indicates the results of densitometric analysis for a 66-kDa band (mean ± SE, n = 4). Relative expression level with respect to the ovary is shown in %. From left to right: skeletal muscle, uterus, lung, adipose tissue, kidney, and myocardium. b Relative expression (to ovary) of whole cell and nonnuclear ER proteins obtained from C2C12 mouse myoblasts (n = 3)

Article Snippet: C2C12 murine skeletal muscle cell line [ 20 ], obtained from Dainippon Pharmaceutical Ltd., was routinely cultured in Dulbecco’s Modified Eagle’s Medium F12 (DMEM/F12) containing 10% heat-inactivated (30 min, 56°C) fetal bovine serum (FBS).

Techniques: Western Blot, Expressing

Time course of ER mRNA and protein expression during E2 exposure. a Representative of C2C12 mRNA transcripts for ER and GAPDH at several time points after exposure to E2 (10−8 M) (upper). The mRNA level of ER relative to GAPDH is averaged from five individual experiments and displayed as mean ± SE (statistically significant). At 0, 0.5, 1, 3, and 5 h (lower). b Proteins were extracted from nucleus-free C2C12 cells exposed to E2 (10−8 M) for varying periods of time (from left: 0, 1, 3, 4, 6, 12, and 24 h) and immunoblotted with the ER antibody. Immunodensity in a 66-kDa band is quantified by densitometry and presented as the % increase of control (time 0). Symbols and bars represent mean ± SE (n = 5); statistically significant

Journal: The Journal of Physiological Sciences : JPS

Article Title: 17β-Estradiol-induced enhancement of estrogen receptor biosynthesis via MAPK pathway in mouse skeletal muscle myoblasts

doi: 10.1007/s12576-009-0023-0

Figure Lengend Snippet: Time course of ER mRNA and protein expression during E2 exposure. a Representative of C2C12 mRNA transcripts for ER and GAPDH at several time points after exposure to E2 (10−8 M) (upper). The mRNA level of ER relative to GAPDH is averaged from five individual experiments and displayed as mean ± SE (statistically significant). At 0, 0.5, 1, 3, and 5 h (lower). b Proteins were extracted from nucleus-free C2C12 cells exposed to E2 (10−8 M) for varying periods of time (from left: 0, 1, 3, 4, 6, 12, and 24 h) and immunoblotted with the ER antibody. Immunodensity in a 66-kDa band is quantified by densitometry and presented as the % increase of control (time 0). Symbols and bars represent mean ± SE (n = 5); statistically significant

Article Snippet: C2C12 murine skeletal muscle cell line [ 20 ], obtained from Dainippon Pharmaceutical Ltd., was routinely cultured in Dulbecco’s Modified Eagle’s Medium F12 (DMEM/F12) containing 10% heat-inactivated (30 min, 56°C) fetal bovine serum (FBS).

Techniques: Expressing, Control

Dose–dependent effects of 17β-estradiol on the expression of total and nonnuclear ER. Representative immunoreactive bands for total ER in C2C12 myoblasts (upper panel) and the relative expression of total ER protein (a) or nonnuclear ER protein (b) quantified by densitometric analysis (lower panel); after treatment with either vehicle (control), different concentrations of 17β-estradiol (10−12–10−5 M) in a and (10−10–10−6 M) in b for 15 h. c Shows the relative expression of total ER protein after treatment with vehicle (control), 17β-estradiol (10−8 M), and BSA-conjugated E2 (10−8 M) for 15 h. Each bar is normalized to the control (far left) and expressed as %. Columns and bars indicate mean ± SE (n = 3 in a, n = 4 in b, and n = 5 in c). *P < 0.01, ☆ P < 0.05 compared with control values, determined using Student’s t test

Journal: The Journal of Physiological Sciences : JPS

Article Title: 17β-Estradiol-induced enhancement of estrogen receptor biosynthesis via MAPK pathway in mouse skeletal muscle myoblasts

doi: 10.1007/s12576-009-0023-0

Figure Lengend Snippet: Dose–dependent effects of 17β-estradiol on the expression of total and nonnuclear ER. Representative immunoreactive bands for total ER in C2C12 myoblasts (upper panel) and the relative expression of total ER protein (a) or nonnuclear ER protein (b) quantified by densitometric analysis (lower panel); after treatment with either vehicle (control), different concentrations of 17β-estradiol (10−12–10−5 M) in a and (10−10–10−6 M) in b for 15 h. c Shows the relative expression of total ER protein after treatment with vehicle (control), 17β-estradiol (10−8 M), and BSA-conjugated E2 (10−8 M) for 15 h. Each bar is normalized to the control (far left) and expressed as %. Columns and bars indicate mean ± SE (n = 3 in a, n = 4 in b, and n = 5 in c). *P < 0.01, ☆ P < 0.05 compared with control values, determined using Student’s t test

Article Snippet: C2C12 murine skeletal muscle cell line [ 20 ], obtained from Dainippon Pharmaceutical Ltd., was routinely cultured in Dulbecco’s Modified Eagle’s Medium F12 (DMEM/F12) containing 10% heat-inactivated (30 min, 56°C) fetal bovine serum (FBS).

Techniques: Expressing, Control

Influence of 17-βestradiol on C2C12 proliferation. C2C12 cells were grown in the serum-free medium for 48 h, which was then changed to ones with or without E2 (10−8 M) in 1% FBS (open triangle, closed triangle) or 10% FBS (open circle, closed circle). The rate of cell growth is shown as the cell number at every 24 h relative to 0 h. Symbols and bars represent the mean ± SE (n = 5)

Journal: The Journal of Physiological Sciences : JPS

Article Title: 17β-Estradiol-induced enhancement of estrogen receptor biosynthesis via MAPK pathway in mouse skeletal muscle myoblasts

doi: 10.1007/s12576-009-0023-0

Figure Lengend Snippet: Influence of 17-βestradiol on C2C12 proliferation. C2C12 cells were grown in the serum-free medium for 48 h, which was then changed to ones with or without E2 (10−8 M) in 1% FBS (open triangle, closed triangle) or 10% FBS (open circle, closed circle). The rate of cell growth is shown as the cell number at every 24 h relative to 0 h. Symbols and bars represent the mean ± SE (n = 5)

Article Snippet: C2C12 murine skeletal muscle cell line [ 20 ], obtained from Dainippon Pharmaceutical Ltd., was routinely cultured in Dulbecco’s Modified Eagle’s Medium F12 (DMEM/F12) containing 10% heat-inactivated (30 min, 56°C) fetal bovine serum (FBS).

Techniques:

Effects of ER antagonists on E2-induced whole-cell and non-nuclear ER increase. C2C12 cells were treated with E2 (10−8 M) in the absence or presence of either tamoxifen or ICI 182,780 (10−7 M; a total ER) or (10−5 M; b non-nuclear fractions) for 15 h. Tamoxifen or ICI 182,780 was added to the culture medium 30 min prior to the administration of E2. Total and nonnuclear fractions of ER were prepared as described in “Materials and methods” and subjected to Western blotting. Histograms represent the extent of inhibition of E2-induced ER increase in total a and nonnuclear b fractions, which are expressed as the percentage of control (no stimulation), averaged from five separate experiments (mean ± SE). *P < 0.01, compared with control values, determined using Student’s t test

Journal: The Journal of Physiological Sciences : JPS

Article Title: 17β-Estradiol-induced enhancement of estrogen receptor biosynthesis via MAPK pathway in mouse skeletal muscle myoblasts

doi: 10.1007/s12576-009-0023-0

Figure Lengend Snippet: Effects of ER antagonists on E2-induced whole-cell and non-nuclear ER increase. C2C12 cells were treated with E2 (10−8 M) in the absence or presence of either tamoxifen or ICI 182,780 (10−7 M; a total ER) or (10−5 M; b non-nuclear fractions) for 15 h. Tamoxifen or ICI 182,780 was added to the culture medium 30 min prior to the administration of E2. Total and nonnuclear fractions of ER were prepared as described in “Materials and methods” and subjected to Western blotting. Histograms represent the extent of inhibition of E2-induced ER increase in total a and nonnuclear b fractions, which are expressed as the percentage of control (no stimulation), averaged from five separate experiments (mean ± SE). *P < 0.01, compared with control values, determined using Student’s t test

Article Snippet: C2C12 murine skeletal muscle cell line [ 20 ], obtained from Dainippon Pharmaceutical Ltd., was routinely cultured in Dulbecco’s Modified Eagle’s Medium F12 (DMEM/F12) containing 10% heat-inactivated (30 min, 56°C) fetal bovine serum (FBS).

Techniques: Western Blot, Inhibition, Control

Effects of 17β-estradiol and/or TPA on ER de novo synthesis. In the absence (control) or presence of 17β-estradiol (10−8 M) and/or TPA (10−6 M), C2C12 cells were labeled with [35S] methionine. At the time points indicated in the figure, the total ER fractions were prepared and subjected to selective immunoprecipitation for ER. The immunoprecipitates were analyzed by SDS-PAGE and image scanning. Data are the mean ± SE from three separate experiments. Left panel time course of 35methionine incorporation in ER. Right panel rates of the increase at 5 h by the relative ratio of control. *P < 0.01, compared with control values, determined using Student’s t test. ☆ P < 0.01, compared with E2 values, determined using Student’s t test

Journal: The Journal of Physiological Sciences : JPS

Article Title: 17β-Estradiol-induced enhancement of estrogen receptor biosynthesis via MAPK pathway in mouse skeletal muscle myoblasts

doi: 10.1007/s12576-009-0023-0

Figure Lengend Snippet: Effects of 17β-estradiol and/or TPA on ER de novo synthesis. In the absence (control) or presence of 17β-estradiol (10−8 M) and/or TPA (10−6 M), C2C12 cells were labeled with [35S] methionine. At the time points indicated in the figure, the total ER fractions were prepared and subjected to selective immunoprecipitation for ER. The immunoprecipitates were analyzed by SDS-PAGE and image scanning. Data are the mean ± SE from three separate experiments. Left panel time course of 35methionine incorporation in ER. Right panel rates of the increase at 5 h by the relative ratio of control. *P < 0.01, compared with control values, determined using Student’s t test. ☆ P < 0.01, compared with E2 values, determined using Student’s t test

Article Snippet: C2C12 murine skeletal muscle cell line [ 20 ], obtained from Dainippon Pharmaceutical Ltd., was routinely cultured in Dulbecco’s Modified Eagle’s Medium F12 (DMEM/F12) containing 10% heat-inactivated (30 min, 56°C) fetal bovine serum (FBS).

Techniques: Control, Labeling, Immunoprecipitation, SDS Page